Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in-vitro and in-vivo
Identifieur interne : 002A37 ( Main/Exploration ); précédent : 002A36; suivant : 002A38Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in-vitro and in-vivo
Auteurs : Susanne Pfefferle [Allemagne] ; Verena Kr Hling ; Vanessa Ditt [Allemagne] ; Klaus Grywna [Allemagne] ; Elke Mühlberger [Allemagne, États-Unis] ; Christian Drosten [Allemagne]Source :
- Virology Journal [ 1743-422X ] ; 2009.
Descripteurs français
- KwdFr :
- Analyse de séquence d'ADN, Animaux, Chromosomes artificiels de bactérie, Cricetinae, Données de séquences moléculaires, Délétion de séquence, Facteurs de virulence (génétique), Facteurs de virulence (physiologie), Humains, Lignée cellulaire, Mesocricetus, Poumon (virologie), Protéines virales (génétique), Protéines virales (physiologie), Réplication virale, Syndrome respiratoire aigu sévère (anatomopathologie), Syndrome respiratoire aigu sévère (virologie), Vecteurs génétiques, Virulence, Virus du SRAS (génétique), Virus du SRAS (pathogénicité).
- MESH :
- anatomopathologie : Syndrome respiratoire aigu sévère.
- génétique : Facteurs de virulence, Protéines virales, Virus du SRAS.
- pathogénicité : Virus du SRAS.
- physiologie : Facteurs de virulence, Protéines virales.
- virologie : Poumon, Syndrome respiratoire aigu sévère.
- Analyse de séquence d'ADN, Animaux, Chromosomes artificiels de bactérie, Cricetinae, Données de séquences moléculaires, Délétion de séquence, Humains, Lignée cellulaire, Mesocricetus, Réplication virale, Vecteurs génétiques, Virulence.
English descriptors
- KwdEn :
- Animals, Cell Line, Chlorocebus aethiops, Chromosomes, Artificial, Bacterial, Cricetinae, Genetic Vectors, Humans, Lung (virology), Mesocricetus, Molecular Sequence Data, SARS Virus (genetics), SARS Virus (pathogenicity), Sequence Analysis, DNA, Sequence Deletion, Severe Acute Respiratory Syndrome (pathology), Severe Acute Respiratory Syndrome (virology), Viral Proteins (genetics), Viral Proteins (physiology), Virulence, Virulence Factors (genetics), Virulence Factors (physiology), Virus Replication.
- MESH :
- chemical , genetics : Viral Proteins, Virulence Factors.
- genetics : SARS Virus.
- pathogenicity : SARS Virus.
- pathology : Severe Acute Respiratory Syndrome.
- chemical , physiology : Viral Proteins, Virulence Factors.
- virology : Lung, Severe Acute Respiratory Syndrome.
- Animals, Cell Line, Chlorocebus aethiops, Chromosomes, Artificial, Bacterial, Cricetinae, Genetic Vectors, Humans, Mesocricetus, Molecular Sequence Data, Sequence Analysis, DNA, Sequence Deletion, Virulence, Virus Replication.
Abstract
During the outbreak of SARS in 2002/3, a prototype virus was isolated from a patient in Frankfurt/Germany (strain Frankfurt-1). As opposed to all other SARS-Coronavirus strains, Frankfurt-1 has a 45-nucleotide deletion in the transmembrane domain of its ORF 7b protein. When over-expressed in HEK 293 cells, the full-length protein but not the variant with the deletion caused interferon beta induction and cleavage of procaspase 3. To study the role of ORF 7b in the context of virus replication, we cloned a full genome cDNA copy of Frankfurt-1 in a bacterial artificial chromosome downstream of a T7 RNA polymerase promoter. Transfection of capped RNA transcribed from this construct yielded infectious virus that was indistinguishable from the original virus isolate. The presumed Frankfurt-1 ancestor with an intact ORF 7b was reconstructed. In CaCo-2 and HUH7 cells, but not in Vero cells, the variant carrying the ORF 7b deletion had a replicative advantage against the parental virus (4- and 6-fold increase of virus RNA in supernatant, respectively). This effect was neither associated with changes in the induction or secretion of type I interferon, nor with altered induction of apoptosis in cell culture. However, pretreatment of cells with interferon beta caused the deleted virus to replicate to higher titers than the parental strain (3.4-fold in Vero cells, 7.9-fold in CaCo-2 cells).
In Syrian Golden Hamsters inoculated intranasally with 10e4 plaque forming units of either virus, mean titers of infectious virus and viral RNA in the lungs after 24 h were increased 23- and 94.8-fold, respectively, with the deleted virus. This difference could explain earlier observations of enhanced virulence of Frankfurt-1 in Hamsters as compared to other SARS-Coronavirus reference strains and identifies the SARS-CoV 7b protein as an attenuating factor with the SARS-Coronavirus genome. Because attenuation was focused on the early phase of infection
The online version of this article (doi:10.1186/1743-422X-6-131) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/1743-422X-6-131
PubMed: 19698190
PubMed Central: 2739521
Affiliations:
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Le document en format XML
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<front><div type="abstract" xml:lang="en"><p>During the outbreak of SARS in 2002/3, a prototype virus was isolated from a patient in Frankfurt/Germany (strain Frankfurt-1). As opposed to all other SARS-Coronavirus strains, Frankfurt-1 has a 45-nucleotide deletion in the transmembrane domain of its ORF 7b protein. When over-expressed in HEK 293 cells, the full-length protein but not the variant with the deletion caused interferon beta induction and cleavage of procaspase 3. To study the role of ORF 7b in the context of virus replication, we cloned a full genome cDNA copy of Frankfurt-1 in a bacterial artificial chromosome downstream of a T7 RNA polymerase promoter. Transfection of capped RNA transcribed from this construct yielded infectious virus that was indistinguishable from the original virus isolate. The presumed Frankfurt-1 ancestor with an intact ORF 7b was reconstructed. In CaCo-2 and HUH7 cells, but not in Vero cells, the variant carrying the ORF 7b deletion had a replicative advantage against the parental virus (4- and 6-fold increase of virus RNA in supernatant, respectively). This effect was neither associated with changes in the induction or secretion of type I interferon, nor with altered induction of apoptosis in cell culture. However, pretreatment of cells with interferon beta caused the deleted virus to replicate to higher titers than the parental strain (3.4-fold in Vero cells, 7.9-fold in CaCo-2 cells).</p>
<p>In Syrian Golden Hamsters inoculated intranasally with 10e4 plaque forming units of either virus, mean titers of infectious virus and viral RNA in the lungs after 24 h were increased 23- and 94.8-fold, respectively, with the deleted virus. This difference could explain earlier observations of enhanced virulence of Frankfurt-1 in Hamsters as compared to other SARS-Coronavirus reference strains and identifies the SARS-CoV 7b protein as an attenuating factor with the SARS-Coronavirus genome. Because attenuation was focused on the early phase of infection <italic>in-vivo</italic>
, ORF 7b might have contributed to the delayed accumulation of virus in patients that was suggested to have limited the spread of the SARS epidemic.</p>
<sec><title>Electronic supplementary material</title>
<p>The online version of this article (doi:10.1186/1743-422X-6-131) contains supplementary material, which is available to authorized users.</p>
</sec>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Peiris, Js" uniqKey="Peiris J">JS Peiris</name>
</author>
<author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name>
</author>
<author><name sortKey="Osterhaus, Ad" uniqKey="Osterhaus A">AD Osterhaus</name>
</author>
<author><name sortKey="Stohr, K" uniqKey="Stohr K">K Stohr</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name>
</author>
<author><name sortKey="Gunther, S" uniqKey="Gunther S">S Gunther</name>
</author>
<author><name sortKey="Preiser, W" uniqKey="Preiser W">W Preiser</name>
</author>
<author><name sortKey="Werf, Svan Der" uniqKey="Werf S">Svan der Werf</name>
</author>
<author><name sortKey="Brodt, Hr" uniqKey="Brodt H">HR Brodt</name>
</author>
<author><name sortKey="Becker, S" uniqKey="Becker S">S Becker</name>
</author>
<author><name sortKey="Rabenau, H" uniqKey="Rabenau H">H Rabenau</name>
</author>
<author><name sortKey="Panning, M" uniqKey="Panning M">M Panning</name>
</author>
<author><name sortKey="Kolesnikova, L" uniqKey="Kolesnikova L">L Kolesnikova</name>
</author>
<author><name sortKey="Fouchier, Ra" uniqKey="Fouchier R">RA Fouchier</name>
</author>
<author><name sortKey="Berger, A" uniqKey="Berger A">A Berger</name>
</author>
<author><name sortKey="Burguire, Am" uniqKey="Burguire A">AM Burguire</name>
</author>
<author><name sortKey="Cinatl, C" uniqKey="Cinatl C">C Cinatl</name>
</author>
<author><name sortKey="Eickmann, M" uniqKey="Eickmann M">M Eickmann</name>
</author>
<author><name sortKey="Escriou, N" uniqKey="Escriou N">N Escriou</name>
</author>
<author><name sortKey="Grywna, K" uniqKey="Grywna K">K Grywna</name>
</author>
<author><name sortKey="Kramme, S" uniqKey="Kramme S">S Kramme</name>
</author>
<author><name sortKey="Manuguerra, Jc" uniqKey="Manuguerra J">JC Manuguerra</name>
</author>
<author><name sortKey="Muller, S" uniqKey="Muller S">S Muller</name>
</author>
<author><name sortKey="Rickerts, V" uniqKey="Rickerts V">V Rickerts</name>
</author>
<author><name sortKey="Sturmer, M" uniqKey="Sturmer M">M Sturmer</name>
</author>
<author><name sortKey="Vieth, S" uniqKey="Vieth S">S Vieth</name>
</author>
<author><name sortKey="Klenk, Hd" uniqKey="Klenk H">HD Klenk</name>
</author>
<author><name sortKey="Osterhaus, Ad" uniqKey="Osterhaus A">AD Osterhaus</name>
</author>
<author><name sortKey="Schmitz, H" uniqKey="Schmitz H">H Schmitz</name>
</author>
<author><name sortKey="Doerr, Hw" uniqKey="Doerr H">HW Doerr</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name>
</author>
<author><name sortKey="Preiser, W" uniqKey="Preiser W">W Preiser</name>
</author>
<author><name sortKey="Gunther, S" uniqKey="Gunther S">S Gunther</name>
</author>
<author><name sortKey="Schmitz, H" uniqKey="Schmitz H">H Schmitz</name>
</author>
<author><name sortKey="Doerr, Hw" uniqKey="Doerr H">HW Doerr</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ksiazek, Tg" uniqKey="Ksiazek T">TG Ksiazek</name>
</author>
<author><name sortKey="Erdman, D" uniqKey="Erdman D">D Erdman</name>
</author>
<author><name sortKey="Goldsmith, Cs" uniqKey="Goldsmith C">CS Goldsmith</name>
</author>
<author><name sortKey="Zaki, Sr" uniqKey="Zaki S">SR Zaki</name>
</author>
<author><name sortKey="Peret, T" uniqKey="Peret T">T Peret</name>
</author>
<author><name sortKey="Emery, S" uniqKey="Emery S">S Emery</name>
</author>
<author><name sortKey="Tong, S" uniqKey="Tong S">S Tong</name>
</author>
<author><name sortKey="Urbani, C" uniqKey="Urbani C">C Urbani</name>
</author>
<author><name sortKey="Comer, Ja" uniqKey="Comer J">JA Comer</name>
</author>
<author><name sortKey="Lim, W" uniqKey="Lim W">W Lim</name>
</author>
<author><name sortKey="Rollin, Pe" uniqKey="Rollin P">PE Rollin</name>
</author>
<author><name sortKey="Dowell, Sf" uniqKey="Dowell S">SF Dowell</name>
</author>
<author><name sortKey="Ling, Ae" uniqKey="Ling A">AE Ling</name>
</author>
<author><name sortKey="Humphrey, Cd" uniqKey="Humphrey C">CD Humphrey</name>
</author>
<author><name sortKey="Shieh, Wj" uniqKey="Shieh W">WJ Shieh</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kuiken, T" uniqKey="Kuiken T">T Kuiken</name>
</author>
<author><name sortKey="Fouchier, Ra" uniqKey="Fouchier R">RA Fouchier</name>
</author>
<author><name sortKey="Schutten, M" uniqKey="Schutten M">M Schutten</name>
</author>
<author><name sortKey="Rimmelzwaan, Gf" uniqKey="Rimmelzwaan G">GF Rimmelzwaan</name>
</author>
<author><name sortKey="Van Amerongen, G" uniqKey="Van Amerongen G">G van Amerongen</name>
</author>
<author><name sortKey="Van Riel, D" uniqKey="Van Riel D">D van Riel</name>
</author>
<author><name sortKey="Laman, Jd" uniqKey="Laman J">JD Laman</name>
</author>
<author><name sortKey="De Jong, T" uniqKey="De Jong T">T de Jong</name>
</author>
<author><name sortKey="Van Doornum, G" uniqKey="Van Doornum G">G van Doornum</name>
</author>
<author><name sortKey="Lim, W" uniqKey="Lim W">W Lim</name>
</author>
<author><name sortKey="Ling, Ae" uniqKey="Ling A">AE Ling</name>
</author>
<author><name sortKey="Chan, Pk" uniqKey="Chan P">PK Chan</name>
</author>
<author><name sortKey="Tam, Js" uniqKey="Tam J">JS Tam</name>
</author>
<author><name sortKey="Zambon, Mc" uniqKey="Zambon M">MC Zambon</name>
</author>
<author><name sortKey="Gopal, R" uniqKey="Gopal R">R Gopal</name>
</author>
<author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name>
</author>
<author><name sortKey="Werf, Svan Der" uniqKey="Werf S">Svan der Werf</name>
</author>
<author><name sortKey="Escriou, N" uniqKey="Escriou N">N Escriou</name>
</author>
<author><name sortKey="Manuguerra, Jc" uniqKey="Manuguerra J">JC Manuguerra</name>
</author>
<author><name sortKey="Stohr, K" uniqKey="Stohr K">K Stohr</name>
</author>
<author><name sortKey="Peiris, Js" uniqKey="Peiris J">JS Peiris</name>
</author>
<author><name sortKey="Osterhaus, Ad" uniqKey="Osterhaus A">AD Osterhaus</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rota, Pa" uniqKey="Rota P">PA Rota</name>
</author>
<author><name sortKey="Oberste, Ms" uniqKey="Oberste M">MS Oberste</name>
</author>
<author><name sortKey="Monroe, Ss" uniqKey="Monroe S">SS Monroe</name>
</author>
<author><name sortKey="Nix, Wa" uniqKey="Nix W">WA Nix</name>
</author>
<author><name sortKey="Campagnoli, R" uniqKey="Campagnoli R">R Campagnoli</name>
</author>
<author><name sortKey="Icenogle, Jp" uniqKey="Icenogle J">JP Icenogle</name>
</author>
<author><name sortKey="Penaranda, S" uniqKey="Penaranda S">S Penaranda</name>
</author>
<author><name sortKey="Bankamp, B" uniqKey="Bankamp B">B Bankamp</name>
</author>
<author><name sortKey="Maher, K" uniqKey="Maher K">K Maher</name>
</author>
<author><name sortKey="Chen, Mh" uniqKey="Chen M">MH Chen</name>
</author>
<author><name sortKey="Tong, S" uniqKey="Tong S">S Tong</name>
</author>
<author><name sortKey="Tamin, A" uniqKey="Tamin A">A Tamin</name>
</author>
<author><name sortKey="Lowe, L" uniqKey="Lowe L">L Lowe</name>
</author>
<author><name sortKey="Frace, M" uniqKey="Frace M">M Frace</name>
</author>
<author><name sortKey="Derisi, Jl" uniqKey="Derisi J">JL DeRisi</name>
</author>
<author><name sortKey="Chen, Q" uniqKey="Chen Q">Q Chen</name>
</author>
<author><name sortKey="Wang, D" uniqKey="Wang D">D Wang</name>
</author>
<author><name sortKey="Erdman, Dd" uniqKey="Erdman D">DD Erdman</name>
</author>
<author><name sortKey="Peret, Tc" uniqKey="Peret T">TC Peret</name>
</author>
<author><name sortKey="Burns, C" uniqKey="Burns C">C Burns</name>
</author>
<author><name sortKey="Ksiazek, Tg" uniqKey="Ksiazek T">TG Ksiazek</name>
</author>
<author><name sortKey="Rollin, Pe" uniqKey="Rollin P">PE Rollin</name>
</author>
<author><name sortKey="Sanchez, A" uniqKey="Sanchez A">A Sanchez</name>
</author>
<author><name sortKey="Liffick, S" uniqKey="Liffick S">S Liffick</name>
</author>
<author><name sortKey="Holloway, B" uniqKey="Holloway B">B Holloway</name>
</author>
<author><name sortKey="Limor, K" uniqKey="Limor K">K Limor</name>
</author>
<author><name sortKey="Mccaustland, K" uniqKey="Mccaustland K">K McCaustland</name>
</author>
<author><name sortKey="Olsen Rasmussen, M" uniqKey="Olsen Rasmussen M">M Olsen-Rasmussen</name>
</author>
<author><name sortKey="Fouchier, R" uniqKey="Fouchier R">R Fouchier</name>
</author>
<author><name sortKey="Gunther, S" uniqKey="Gunther S">S Gunther</name>
</author>
<author><name sortKey="Osterhaus, Ad" uniqKey="Osterhaus A">AD Osterhaus</name>
</author>
<author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name>
</author>
<author><name sortKey="Pallansch, Ma" uniqKey="Pallansch M">MA Pallansch</name>
</author>
<author><name sortKey="Anderson, Lj" uniqKey="Anderson L">LJ Anderson</name>
</author>
<author><name sortKey="Bellini, Wj" uniqKey="Bellini W">WJ Bellini</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name>
</author>
<author><name sortKey="Zheng, Bj" uniqKey="Zheng B">BJ Zheng</name>
</author>
<author><name sortKey="He, Yq" uniqKey="He Y">YQ He</name>
</author>
<author><name sortKey="Liu, Xl" uniqKey="Liu X">XL Liu</name>
</author>
<author><name sortKey="Zhuang, Zx" uniqKey="Zhuang Z">ZX Zhuang</name>
</author>
<author><name sortKey="Cheung, Cl" uniqKey="Cheung C">CL Cheung</name>
</author>
<author><name sortKey="Luo, Sw" uniqKey="Luo S">SW Luo</name>
</author>
<author><name sortKey="Li, Ph" uniqKey="Li P">PH Li</name>
</author>
<author><name sortKey="Zhang, Lj" uniqKey="Zhang L">LJ Zhang</name>
</author>
<author><name sortKey="Guan, Yj" uniqKey="Guan Y">YJ Guan</name>
</author>
<author><name sortKey="Butt, Km" uniqKey="Butt K">KM Butt</name>
</author>
<author><name sortKey="Wong, Kl" uniqKey="Wong K">KL Wong</name>
</author>
<author><name sortKey="Chan, Kw" uniqKey="Chan K">KW Chan</name>
</author>
<author><name sortKey="Lim, W" uniqKey="Lim W">W Lim</name>
</author>
<author><name sortKey="Shortridge, Kf" uniqKey="Shortridge K">KF Shortridge</name>
</author>
<author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name>
</author>
<author><name sortKey="Peiris, Js" uniqKey="Peiris J">JS Peiris</name>
</author>
<author><name sortKey="Poon, Ll" uniqKey="Poon L">LL Poon</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name>
</author>
<author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name>
</author>
<author><name sortKey="Li, Ks" uniqKey="Li K">KS Li</name>
</author>
<author><name sortKey="Huang, Y" uniqKey="Huang Y">Y Huang</name>
</author>
<author><name sortKey="Tsoi, Hw" uniqKey="Tsoi H">HW Tsoi</name>
</author>
<author><name sortKey="Wong, Bh" uniqKey="Wong B">BH Wong</name>
</author>
<author><name sortKey="Wong, Ss" uniqKey="Wong S">SS Wong</name>
</author>
<author><name sortKey="Leung, Sy" uniqKey="Leung S">SY Leung</name>
</author>
<author><name sortKey="Chan, Kh" uniqKey="Chan K">KH Chan</name>
</author>
<author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Li, W" uniqKey="Li W">W Li</name>
</author>
<author><name sortKey="Shi, Z" uniqKey="Shi Z">Z Shi</name>
</author>
<author><name sortKey="Yu, M" uniqKey="Yu M">M Yu</name>
</author>
<author><name sortKey="Ren, W" uniqKey="Ren W">W Ren</name>
</author>
<author><name sortKey="Smith, C" uniqKey="Smith C">C Smith</name>
</author>
<author><name sortKey="Epstein, Jh" uniqKey="Epstein J">JH Epstein</name>
</author>
<author><name sortKey="Wang, H" uniqKey="Wang H">H Wang</name>
</author>
<author><name sortKey="Crameri, G" uniqKey="Crameri G">G Crameri</name>
</author>
<author><name sortKey="Hu, Z" uniqKey="Hu Z">Z Hu</name>
</author>
<author><name sortKey="Zhang, H" uniqKey="Zhang H">H Zhang</name>
</author>
<author><name sortKey="Zhang, J" uniqKey="Zhang J">J Zhang</name>
</author>
<author><name sortKey="Mceachern, J" uniqKey="Mceachern J">J McEachern</name>
</author>
<author><name sortKey="Field, H" uniqKey="Field H">H Field</name>
</author>
<author><name sortKey="Daszak, P" uniqKey="Daszak P">P Daszak</name>
</author>
<author><name sortKey="Eaton, Bt" uniqKey="Eaton B">BT Eaton</name>
</author>
<author><name sortKey="Zhang, S" uniqKey="Zhang S">S Zhang</name>
</author>
<author><name sortKey="Wang, Lf" uniqKey="Wang L">LF Wang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Poon, Ll" uniqKey="Poon L">LL Poon</name>
</author>
<author><name sortKey="Chu, Dk" uniqKey="Chu D">DK Chu</name>
</author>
<author><name sortKey="Chan, Kh" uniqKey="Chan K">KH Chan</name>
</author>
<author><name sortKey="Wong, Ok" uniqKey="Wong O">OK Wong</name>
</author>
<author><name sortKey="Ellis, Tm" uniqKey="Ellis T">TM Ellis</name>
</author>
<author><name sortKey="Leung, Yh" uniqKey="Leung Y">YH Leung</name>
</author>
<author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name>
</author>
<author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name>
</author>
<author><name sortKey="Suen, Ky" uniqKey="Suen K">KY Suen</name>
</author>
<author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name>
</author>
<author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name>
</author>
<author><name sortKey="Peiris, Js" uniqKey="Peiris J">JS Peiris</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tang, Xc" uniqKey="Tang X">XC Tang</name>
</author>
<author><name sortKey="Zhang, Jx" uniqKey="Zhang J">JX Zhang</name>
</author>
<author><name sortKey="Zhang, Sy" uniqKey="Zhang S">SY Zhang</name>
</author>
<author><name sortKey="Wang, P" uniqKey="Wang P">P Wang</name>
</author>
<author><name sortKey="Fan, Xh" uniqKey="Fan X">XH Fan</name>
</author>
<author><name sortKey="Li, Lf" uniqKey="Li L">LF Li</name>
</author>
<author><name sortKey="Li, G" uniqKey="Li G">G Li</name>
</author>
<author><name sortKey="Dong, Bq" uniqKey="Dong B">BQ Dong</name>
</author>
<author><name sortKey="Liu, W" uniqKey="Liu W">W Liu</name>
</author>
<author><name sortKey="Cheung, Cl" uniqKey="Cheung C">CL Cheung</name>
</author>
<author><name sortKey="Xu, Km" uniqKey="Xu K">KM Xu</name>
</author>
<author><name sortKey="Song, Wj" uniqKey="Song W">WJ Song</name>
</author>
<author><name sortKey="Vijaykrishna, D" uniqKey="Vijaykrishna D">D Vijaykrishna</name>
</author>
<author><name sortKey="Poon, Ll" uniqKey="Poon L">LL Poon</name>
</author>
<author><name sortKey="Peiris, Js" uniqKey="Peiris J">JS Peiris</name>
</author>
<author><name sortKey="Smith, Gj" uniqKey="Smith G">GJ Smith</name>
</author>
<author><name sortKey="Chen, H" uniqKey="Chen H">H Chen</name>
</author>
<author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wang, Lf" uniqKey="Wang L">LF Wang</name>
</author>
<author><name sortKey="Shi, Z" uniqKey="Shi Z">Z Shi</name>
</author>
<author><name sortKey="Zhang, S" uniqKey="Zhang S">S Zhang</name>
</author>
<author><name sortKey="Field, H" uniqKey="Field H">H Field</name>
</author>
<author><name sortKey="Daszak, P" uniqKey="Daszak P">P Daszak</name>
</author>
<author><name sortKey="Eaton, Bt" uniqKey="Eaton B">BT Eaton</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cavanagh, D" uniqKey="Cavanagh D">D Cavanagh</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Herrewegh, Aa" uniqKey="Herrewegh A">AA Herrewegh</name>
</author>
<author><name sortKey="Smeenk, I" uniqKey="Smeenk I">I Smeenk</name>
</author>
<author><name sortKey="Horzinek, Mc" uniqKey="Horzinek M">MC Horzinek</name>
</author>
<author><name sortKey="Rottier, Pj" uniqKey="Rottier P">PJ Rottier</name>
</author>
<author><name sortKey="De Groot, Rj" uniqKey="De Groot R">RJ de Groot</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Saif, Lj" uniqKey="Saif L">LJ Saif</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Vijgen, L" uniqKey="Vijgen L">L Vijgen</name>
</author>
<author><name sortKey="Keyaerts, E" uniqKey="Keyaerts E">E Keyaerts</name>
</author>
<author><name sortKey="Moes, E" uniqKey="Moes E">E Moes</name>
</author>
<author><name sortKey="Thoelen, I" uniqKey="Thoelen I">I Thoelen</name>
</author>
<author><name sortKey="Wollants, E" uniqKey="Wollants E">E Wollants</name>
</author>
<author><name sortKey="Lemey, P" uniqKey="Lemey P">P Lemey</name>
</author>
<author><name sortKey="Vandamme, Am" uniqKey="Vandamme A">AM Vandamme</name>
</author>
<author><name sortKey="Van Ranst, M" uniqKey="Van Ranst M">M Van Ranst</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Frieman, Mb" uniqKey="Frieman M">MB Frieman</name>
</author>
<author><name sortKey="Yount, B" uniqKey="Yount B">B Yount</name>
</author>
<author><name sortKey="Sims, Ac" uniqKey="Sims A">AC Sims</name>
</author>
<author><name sortKey="Deming, Dj" uniqKey="Deming D">DJ Deming</name>
</author>
<author><name sortKey="Morrison, Te" uniqKey="Morrison T">TE Morrison</name>
</author>
<author><name sortKey="Sparks, J" uniqKey="Sparks J">J Sparks</name>
</author>
<author><name sortKey="Denison, M" uniqKey="Denison M">M Denison</name>
</author>
<author><name sortKey="Heise, M" uniqKey="Heise M">M Heise</name>
</author>
<author><name sortKey="Baric, Rs" uniqKey="Baric R">RS Baric</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Schaecher, Sr" uniqKey="Schaecher S">SR Schaecher</name>
</author>
<author><name sortKey="Touchette, E" uniqKey="Touchette E">E Touchette</name>
</author>
<author><name sortKey="Schriewer, J" uniqKey="Schriewer J">J Schriewer</name>
</author>
<author><name sortKey="Buller, Rm" uniqKey="Buller R">RM Buller</name>
</author>
<author><name sortKey="Pekosz, A" uniqKey="Pekosz A">A Pekosz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yount, B" uniqKey="Yount B">B Yount</name>
</author>
<author><name sortKey="Roberts, Rs" uniqKey="Roberts R">RS Roberts</name>
</author>
<author><name sortKey="Sims, Ac" uniqKey="Sims A">AC Sims</name>
</author>
<author><name sortKey="Deming, D" uniqKey="Deming D">D Deming</name>
</author>
<author><name sortKey="Frieman, Mb" uniqKey="Frieman M">MB Frieman</name>
</author>
<author><name sortKey="Sparks, J" uniqKey="Sparks J">J Sparks</name>
</author>
<author><name sortKey="Denison, Mr" uniqKey="Denison M">MR Denison</name>
</author>
<author><name sortKey="Davis, N" uniqKey="Davis N">N Davis</name>
</author>
<author><name sortKey="Baric, Rs" uniqKey="Baric R">RS Baric</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Snijder, Ej" uniqKey="Snijder E">EJ Snijder</name>
</author>
<author><name sortKey="Bredenbeek, Pj" uniqKey="Bredenbeek P">PJ Bredenbeek</name>
</author>
<author><name sortKey="Dobbe, Jc" uniqKey="Dobbe J">JC Dobbe</name>
</author>
<author><name sortKey="Thiel, V" uniqKey="Thiel V">V Thiel</name>
</author>
<author><name sortKey="Ziebuhr, J" uniqKey="Ziebuhr J">J Ziebuhr</name>
</author>
<author><name sortKey="Poon, Ll" uniqKey="Poon L">LL Poon</name>
</author>
<author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name>
</author>
<author><name sortKey="Rozanov, M" uniqKey="Rozanov M">M Rozanov</name>
</author>
<author><name sortKey="Spaan, Wj" uniqKey="Spaan W">WJ Spaan</name>
</author>
<author><name sortKey="Gorbalenya, Ae" uniqKey="Gorbalenya A">AE Gorbalenya</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="De Haan, Ca" uniqKey="De Haan C">CA de Haan</name>
</author>
<author><name sortKey="Masters, Ps" uniqKey="Masters P">PS Masters</name>
</author>
<author><name sortKey="Shen, X" uniqKey="Shen X">X Shen</name>
</author>
<author><name sortKey="Weiss, S" uniqKey="Weiss S">S Weiss</name>
</author>
<author><name sortKey="Rottier, Pj" uniqKey="Rottier P">PJ Rottier</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Haijema, Bj" uniqKey="Haijema B">BJ Haijema</name>
</author>
<author><name sortKey="Volders, H" uniqKey="Volders H">H Volders</name>
</author>
<author><name sortKey="Rottier, Pj" uniqKey="Rottier P">PJ Rottier</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Herrewegh, Aa" uniqKey="Herrewegh A">AA Herrewegh</name>
</author>
<author><name sortKey="Vennema, H" uniqKey="Vennema H">H Vennema</name>
</author>
<author><name sortKey="Horzinek, Mc" uniqKey="Horzinek M">MC Horzinek</name>
</author>
<author><name sortKey="Rottier, Pj" uniqKey="Rottier P">PJ Rottier</name>
</author>
<author><name sortKey="De Groot, Rj" uniqKey="De Groot R">RJ de Groot</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ortego, J" uniqKey="Ortego J">J Ortego</name>
</author>
<author><name sortKey="Sola, I" uniqKey="Sola I">I Sola</name>
</author>
<author><name sortKey="Almazan, F" uniqKey="Almazan F">F Almazan</name>
</author>
<author><name sortKey="Ceriani, Je" uniqKey="Ceriani J">JE Ceriani</name>
</author>
<author><name sortKey="Riquelme, C" uniqKey="Riquelme C">C Riquelme</name>
</author>
<author><name sortKey="Balasch, M" uniqKey="Balasch M">M Balasch</name>
</author>
<author><name sortKey="Plana, J" uniqKey="Plana J">J Plana</name>
</author>
<author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L Enjuanes</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chinese, Smec" uniqKey="Chinese S">SMEC Chinese</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chiu, Rw" uniqKey="Chiu R">RW Chiu</name>
</author>
<author><name sortKey="Chim, Ss" uniqKey="Chim S">SS Chim</name>
</author>
<author><name sortKey="Tong, Yk" uniqKey="Tong Y">YK Tong</name>
</author>
<author><name sortKey="Fung, Ks" uniqKey="Fung K">KS Fung</name>
</author>
<author><name sortKey="Chan, Pk" uniqKey="Chan P">PK Chan</name>
</author>
<author><name sortKey="Zhao, Gp" uniqKey="Zhao G">GP Zhao</name>
</author>
<author><name sortKey="Lo, Ym" uniqKey="Lo Y">YM Lo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tang, Jw" uniqKey="Tang J">JW Tang</name>
</author>
<author><name sortKey="Cheung, Jl" uniqKey="Cheung J">JL Cheung</name>
</author>
<author><name sortKey="Chu, Im" uniqKey="Chu I">IM Chu</name>
</author>
<author><name sortKey="Sung, Jj" uniqKey="Sung J">JJ Sung</name>
</author>
<author><name sortKey="Peiris, M" uniqKey="Peiris M">M Peiris</name>
</author>
<author><name sortKey="Chan, Pk" uniqKey="Chan P">PK Chan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rice, Cm" uniqKey="Rice C">CM Rice</name>
</author>
<author><name sortKey="Levis, R" uniqKey="Levis R">R Levis</name>
</author>
<author><name sortKey="Strauss, Jh" uniqKey="Strauss J">JH Strauss</name>
</author>
<author><name sortKey="Huang, Hv" uniqKey="Huang H">HV Huang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Semler, Bl" uniqKey="Semler B">BL Semler</name>
</author>
<author><name sortKey="Dorner, Aj" uniqKey="Dorner A">AJ Dorner</name>
</author>
<author><name sortKey="Wimmer, E" uniqKey="Wimmer E">E Wimmer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Werf, Svan Der" uniqKey="Werf S">Svan der Werf</name>
</author>
<author><name sortKey="Bradley, J" uniqKey="Bradley J">J Bradley</name>
</author>
<author><name sortKey="Wimmer, E" uniqKey="Wimmer E">E Wimmer</name>
</author>
<author><name sortKey="Studier, Fw" uniqKey="Studier F">FW Studier</name>
</author>
<author><name sortKey="Dunn, Jj" uniqKey="Dunn J">JJ Dunn</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yount, B" uniqKey="Yount B">B Yount</name>
</author>
<author><name sortKey="Curtis, Km" uniqKey="Curtis K">KM Curtis</name>
</author>
<author><name sortKey="Baric, Rs" uniqKey="Baric R">RS Baric</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yount, B" uniqKey="Yount B">B Yount</name>
</author>
<author><name sortKey="Curtis, Km" uniqKey="Curtis K">KM Curtis</name>
</author>
<author><name sortKey="Fritz, Ea" uniqKey="Fritz E">EA Fritz</name>
</author>
<author><name sortKey="Hensley, Le" uniqKey="Hensley L">LE Hensley</name>
</author>
<author><name sortKey="Jahrling, Pb" uniqKey="Jahrling P">PB Jahrling</name>
</author>
<author><name sortKey="Prentice, E" uniqKey="Prentice E">E Prentice</name>
</author>
<author><name sortKey="Denison, Mr" uniqKey="Denison M">MR Denison</name>
</author>
<author><name sortKey="Geisbert, Tw" uniqKey="Geisbert T">TW Geisbert</name>
</author>
<author><name sortKey="Baric, Rs" uniqKey="Baric R">RS Baric</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yount, B" uniqKey="Yount B">B Yount</name>
</author>
<author><name sortKey="Denison, Mr" uniqKey="Denison M">MR Denison</name>
</author>
<author><name sortKey="Weiss, Sr" uniqKey="Weiss S">SR Weiss</name>
</author>
<author><name sortKey="Baric, Rs" uniqKey="Baric R">RS Baric</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Thiel, V" uniqKey="Thiel V">V Thiel</name>
</author>
<author><name sortKey="Herold, J" uniqKey="Herold J">J Herold</name>
</author>
<author><name sortKey="Schelle, B" uniqKey="Schelle B">B Schelle</name>
</author>
<author><name sortKey="Siddell, Sg" uniqKey="Siddell S">SG Siddell</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Thiel, V" uniqKey="Thiel V">V Thiel</name>
</author>
<author><name sortKey="Siddell, Sg" uniqKey="Siddell S">SG Siddell</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Almazan, F" uniqKey="Almazan F">F Almazan</name>
</author>
<author><name sortKey="Dediego, Ml" uniqKey="Dediego M">ML Dediego</name>
</author>
<author><name sortKey="Galan, C" uniqKey="Galan C">C Galan</name>
</author>
<author><name sortKey="Escors, D" uniqKey="Escors D">D Escors</name>
</author>
<author><name sortKey="Alvarez, E" uniqKey="Alvarez E">E Alvarez</name>
</author>
<author><name sortKey="Ortego, J" uniqKey="Ortego J">J Ortego</name>
</author>
<author><name sortKey="Sola, I" uniqKey="Sola I">I Sola</name>
</author>
<author><name sortKey="Zuniga, S" uniqKey="Zuniga S">S Zuniga</name>
</author>
<author><name sortKey="Alonso, S" uniqKey="Alonso S">S Alonso</name>
</author>
<author><name sortKey="Moreno, Jl" uniqKey="Moreno J">JL Moreno</name>
</author>
<author><name sortKey="Nogales, A" uniqKey="Nogales A">A Nogales</name>
</author>
<author><name sortKey="Capiscol, C" uniqKey="Capiscol C">C Capiscol</name>
</author>
<author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L Enjuanes</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Almazan, F" uniqKey="Almazan F">F Almazan</name>
</author>
<author><name sortKey="Gonzalez, Jm" uniqKey="Gonzalez J">JM Gonzalez</name>
</author>
<author><name sortKey="Penzes, Z" uniqKey="Penzes Z">Z Penzes</name>
</author>
<author><name sortKey="Izeta, A" uniqKey="Izeta A">A Izeta</name>
</author>
<author><name sortKey="Calvo, E" uniqKey="Calvo E">E Calvo</name>
</author>
<author><name sortKey="Plana Duran, J" uniqKey="Plana Duran J">J Plana-Duran</name>
</author>
<author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L Enjuanes</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gonzalez, Jm" uniqKey="Gonzalez J">JM Gonzalez</name>
</author>
<author><name sortKey="Penzes, Z" uniqKey="Penzes Z">Z Penzes</name>
</author>
<author><name sortKey="Almazan, F" uniqKey="Almazan F">F Almazan</name>
</author>
<author><name sortKey="Calvo, E" uniqKey="Calvo E">E Calvo</name>
</author>
<author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L Enjuanes</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="St Jean, Jr" uniqKey="St Jean J">JR St-Jean</name>
</author>
<author><name sortKey="Desforges, M" uniqKey="Desforges M">M Desforges</name>
</author>
<author><name sortKey="Almazan, F" uniqKey="Almazan F">F Almazan</name>
</author>
<author><name sortKey="Jacomy, H" uniqKey="Jacomy H">H Jacomy</name>
</author>
<author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L Enjuanes</name>
</author>
<author><name sortKey="Talbot, Pj" uniqKey="Talbot P">PJ Talbot</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Schaecher, Sr" uniqKey="Schaecher S">SR Schaecher</name>
</author>
<author><name sortKey="Mackenzie, Jm" uniqKey="Mackenzie J">JM Mackenzie</name>
</author>
<author><name sortKey="Pekosz, A" uniqKey="Pekosz A">A Pekosz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Schaecher, Sr" uniqKey="Schaecher S">SR Schaecher</name>
</author>
<author><name sortKey="Diamond, Ms" uniqKey="Diamond M">MS Diamond</name>
</author>
<author><name sortKey="Pekosz, A" uniqKey="Pekosz A">A Pekosz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cinatl, J" uniqKey="Cinatl J">J Cinatl</name>
</author>
<author><name sortKey="Hoever, G" uniqKey="Hoever G">G Hoever</name>
</author>
<author><name sortKey="Morgenstern, B" uniqKey="Morgenstern B">B Morgenstern</name>
</author>
<author><name sortKey="Preiser, W" uniqKey="Preiser W">W Preiser</name>
</author>
<author><name sortKey="Vogel, Ju" uniqKey="Vogel J">JU Vogel</name>
</author>
<author><name sortKey="Hofmann, Wk" uniqKey="Hofmann W">WK Hofmann</name>
</author>
<author><name sortKey="Bauer, G" uniqKey="Bauer G">G Bauer</name>
</author>
<author><name sortKey="Michaelis, M" uniqKey="Michaelis M">M Michaelis</name>
</author>
<author><name sortKey="Rabenau, Hf" uniqKey="Rabenau H">HF Rabenau</name>
</author>
<author><name sortKey="Doerr, Hw" uniqKey="Doerr H">HW Doerr</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cinatl, J" uniqKey="Cinatl J">J Cinatl</name>
</author>
<author><name sortKey="Michaelis, M" uniqKey="Michaelis M">M Michaelis</name>
</author>
<author><name sortKey="Morgenstern, B" uniqKey="Morgenstern B">B Morgenstern</name>
</author>
<author><name sortKey="Doerr, Hw" uniqKey="Doerr H">HW Doerr</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nagata, N" uniqKey="Nagata N">N Nagata</name>
</author>
<author><name sortKey="Iwata, N" uniqKey="Iwata N">N Iwata</name>
</author>
<author><name sortKey="Hasegawa, H" uniqKey="Hasegawa H">H Hasegawa</name>
</author>
<author><name sortKey="Fukushi, S" uniqKey="Fukushi S">S Fukushi</name>
</author>
<author><name sortKey="Harashima, A" uniqKey="Harashima A">A Harashima</name>
</author>
<author><name sortKey="Sato, Y" uniqKey="Sato Y">Y Sato</name>
</author>
<author><name sortKey="Saijo, M" uniqKey="Saijo M">M Saijo</name>
</author>
<author><name sortKey="Taguchi, F" uniqKey="Taguchi F">F Taguchi</name>
</author>
<author><name sortKey="Morikawa, S" uniqKey="Morikawa S">S Morikawa</name>
</author>
<author><name sortKey="Sata, T" uniqKey="Sata T">T Sata</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nagata, N" uniqKey="Nagata N">N Nagata</name>
</author>
<author><name sortKey="Iwata, N" uniqKey="Iwata N">N Iwata</name>
</author>
<author><name sortKey="Hasegawa, H" uniqKey="Hasegawa H">H Hasegawa</name>
</author>
<author><name sortKey="Fukushi, S" uniqKey="Fukushi S">S Fukushi</name>
</author>
<author><name sortKey="Yokoyama, M" uniqKey="Yokoyama M">M Yokoyama</name>
</author>
<author><name sortKey="Harashima, A" uniqKey="Harashima A">A Harashima</name>
</author>
<author><name sortKey="Sato, Y" uniqKey="Sato Y">Y Sato</name>
</author>
<author><name sortKey="Saijo, M" uniqKey="Saijo M">M Saijo</name>
</author>
<author><name sortKey="Morikawa, S" uniqKey="Morikawa S">S Morikawa</name>
</author>
<author><name sortKey="Sata, T" uniqKey="Sata T">T Sata</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tan, Yj" uniqKey="Tan Y">YJ Tan</name>
</author>
<author><name sortKey="Fielding, Bc" uniqKey="Fielding B">BC Fielding</name>
</author>
<author><name sortKey="Goh, Py" uniqKey="Goh P">PY Goh</name>
</author>
<author><name sortKey="Shen, S" uniqKey="Shen S">S Shen</name>
</author>
<author><name sortKey="Tan, Th" uniqKey="Tan T">TH Tan</name>
</author>
<author><name sortKey="Lim, Sg" uniqKey="Lim S">SG Lim</name>
</author>
<author><name sortKey="Hong, W" uniqKey="Hong W">W Hong</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tan, Yx" uniqKey="Tan Y">YX Tan</name>
</author>
<author><name sortKey="Tan, Th" uniqKey="Tan T">TH Tan</name>
</author>
<author><name sortKey="Lee, Mj" uniqKey="Lee M">MJ Lee</name>
</author>
<author><name sortKey="Tham, Py" uniqKey="Tham P">PY Tham</name>
</author>
<author><name sortKey="Gunalan, V" uniqKey="Gunalan V">V Gunalan</name>
</author>
<author><name sortKey="Druce, J" uniqKey="Druce J">J Druce</name>
</author>
<author><name sortKey="Birch, C" uniqKey="Birch C">C Birch</name>
</author>
<author><name sortKey="Catton, M" uniqKey="Catton M">M Catton</name>
</author>
<author><name sortKey="Fu, Ny" uniqKey="Fu N">NY Fu</name>
</author>
<author><name sortKey="Yu, Vc" uniqKey="Yu V">VC Yu</name>
</author>
<author><name sortKey="Tan, Yj" uniqKey="Tan Y">YJ Tan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Basler, Cf" uniqKey="Basler C">CF Basler</name>
</author>
<author><name sortKey="Wang, X" uniqKey="Wang X">X Wang</name>
</author>
<author><name sortKey="Muhlberger, E" uniqKey="Muhlberger E">E Muhlberger</name>
</author>
<author><name sortKey="Volchkov, V" uniqKey="Volchkov V">V Volchkov</name>
</author>
<author><name sortKey="Paragas, J" uniqKey="Paragas J">J Paragas</name>
</author>
<author><name sortKey="Klenk, Hd" uniqKey="Klenk H">HD Klenk</name>
</author>
<author><name sortKey="Garcia Sastre, A" uniqKey="Garcia Sastre A">A Garcia-Sastre</name>
</author>
<author><name sortKey="Palese, P" uniqKey="Palese P">P Palese</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cardenas, Wb" uniqKey="Cardenas W">WB Cardenas</name>
</author>
<author><name sortKey="Loo, Ym" uniqKey="Loo Y">YM Loo</name>
</author>
<author><name sortKey="Gale, M" uniqKey="Gale M">M Gale</name>
</author>
<author><name sortKey="Hartman, Al" uniqKey="Hartman A">AL Hartman</name>
</author>
<author><name sortKey="Kimberlin, Cr" uniqKey="Kimberlin C">CR Kimberlin</name>
</author>
<author><name sortKey="Martinez Sobrido, L" uniqKey="Martinez Sobrido L">L Martinez-Sobrido</name>
</author>
<author><name sortKey="Saphire, Eo" uniqKey="Saphire E">EO Saphire</name>
</author>
<author><name sortKey="Basler, Cf" uniqKey="Basler C">CF Basler</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Spiegel, M" uniqKey="Spiegel M">M Spiegel</name>
</author>
<author><name sortKey="Weber, F" uniqKey="Weber F">F Weber</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kaufmann, Sh" uniqKey="Kaufmann S">SH Kaufmann</name>
</author>
<author><name sortKey="Desnoyers, S" uniqKey="Desnoyers S">S Desnoyers</name>
</author>
<author><name sortKey="Ottaviano, Y" uniqKey="Ottaviano Y">Y Ottaviano</name>
</author>
<author><name sortKey="Davidson, Ne" uniqKey="Davidson N">NE Davidson</name>
</author>
<author><name sortKey="Poirier, Gg" uniqKey="Poirier G">GG Poirier</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Roberts, A" uniqKey="Roberts A">A Roberts</name>
</author>
<author><name sortKey="Lamirande, Ew" uniqKey="Lamirande E">EW Lamirande</name>
</author>
<author><name sortKey="Vogel, L" uniqKey="Vogel L">L Vogel</name>
</author>
<author><name sortKey="Jackson, Jp" uniqKey="Jackson J">JP Jackson</name>
</author>
<author><name sortKey="Paddock, Cd" uniqKey="Paddock C">CD Paddock</name>
</author>
<author><name sortKey="Guarner, J" uniqKey="Guarner J">J Guarner</name>
</author>
<author><name sortKey="Zaki, Sr" uniqKey="Zaki S">SR Zaki</name>
</author>
<author><name sortKey="Sheahan, T" uniqKey="Sheahan T">T Sheahan</name>
</author>
<author><name sortKey="Baric, R" uniqKey="Baric R">R Baric</name>
</author>
<author><name sortKey="Subbarao, K" uniqKey="Subbarao K">K Subbarao</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Roberts, A" uniqKey="Roberts A">A Roberts</name>
</author>
<author><name sortKey="Vogel, L" uniqKey="Vogel L">L Vogel</name>
</author>
<author><name sortKey="Guarner, J" uniqKey="Guarner J">J Guarner</name>
</author>
<author><name sortKey="Hayes, N" uniqKey="Hayes N">N Hayes</name>
</author>
<author><name sortKey="Murphy, B" uniqKey="Murphy B">B Murphy</name>
</author>
<author><name sortKey="Zaki, S" uniqKey="Zaki S">S Zaki</name>
</author>
<author><name sortKey="Subbarao, K" uniqKey="Subbarao K">K Subbarao</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Racaniello, Vr" uniqKey="Racaniello V">VR Racaniello</name>
</author>
<author><name sortKey="Baltimore, D" uniqKey="Baltimore D">D Baltimore</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Almazan, F" uniqKey="Almazan F">F Almazan</name>
</author>
<author><name sortKey="Galan, C" uniqKey="Galan C">C Galan</name>
</author>
<author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L Enjuanes</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L Enjuanes</name>
</author>
<author><name sortKey="Sola, I" uniqKey="Sola I">I Sola</name>
</author>
<author><name sortKey="Alonso, S" uniqKey="Alonso S">S Alonso</name>
</author>
<author><name sortKey="Escors, D" uniqKey="Escors D">D Escors</name>
</author>
<author><name sortKey="Zuniga, S" uniqKey="Zuniga S">S Zuniga</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ortego, J" uniqKey="Ortego J">J Ortego</name>
</author>
<author><name sortKey="Escors, D" uniqKey="Escors D">D Escors</name>
</author>
<author><name sortKey="Laude, H" uniqKey="Laude H">H Laude</name>
</author>
<author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L Enjuanes</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Devaraj, Sg" uniqKey="Devaraj S">SG Devaraj</name>
</author>
<author><name sortKey="Wang, N" uniqKey="Wang N">N Wang</name>
</author>
<author><name sortKey="Chen, Z" uniqKey="Chen Z">Z Chen</name>
</author>
<author><name sortKey="Chen, Z" uniqKey="Chen Z">Z Chen</name>
</author>
<author><name sortKey="Tseng, M" uniqKey="Tseng M">M Tseng</name>
</author>
<author><name sortKey="Barretto, N" uniqKey="Barretto N">N Barretto</name>
</author>
<author><name sortKey="Lin, R" uniqKey="Lin R">R Lin</name>
</author>
<author><name sortKey="Peters, Cj" uniqKey="Peters C">CJ Peters</name>
</author>
<author><name sortKey="Tseng, Ct" uniqKey="Tseng C">CT Tseng</name>
</author>
<author><name sortKey="Baker, Sc" uniqKey="Baker S">SC Baker</name>
</author>
<author><name sortKey="Li, K" uniqKey="Li K">K Li</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zust, R" uniqKey="Zust R">R Zust</name>
</author>
<author><name sortKey="Cervantes Barragan, L" uniqKey="Cervantes Barragan L">L Cervantes-Barragan</name>
</author>
<author><name sortKey="Kuri, T" uniqKey="Kuri T">T Kuri</name>
</author>
<author><name sortKey="Blakqori, G" uniqKey="Blakqori G">G Blakqori</name>
</author>
<author><name sortKey="Weber, F" uniqKey="Weber F">F Weber</name>
</author>
<author><name sortKey="Ludewig, B" uniqKey="Ludewig B">B Ludewig</name>
</author>
<author><name sortKey="Thiel, V" uniqKey="Thiel V">V Thiel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Versteeg, Ga" uniqKey="Versteeg G">GA Versteeg</name>
</author>
<author><name sortKey="Bredenbeek, Pj" uniqKey="Bredenbeek P">PJ Bredenbeek</name>
</author>
<author><name sortKey="Worm, Shvan Den" uniqKey="Worm S">SHvan den Worm</name>
</author>
<author><name sortKey="Spaan, Wj" uniqKey="Spaan W">WJ Spaan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kopecky Bromberg, Sa" uniqKey="Kopecky Bromberg S">SA Kopecky-Bromberg</name>
</author>
<author><name sortKey="Martinez Sobrido, L" uniqKey="Martinez Sobrido L">L Martinez-Sobrido</name>
</author>
<author><name sortKey="Frieman, M" uniqKey="Frieman M">M Frieman</name>
</author>
<author><name sortKey="Baric, Ra" uniqKey="Baric R">RA Baric</name>
</author>
<author><name sortKey="Palese, P" uniqKey="Palese P">P Palese</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Frieman, M" uniqKey="Frieman M">M Frieman</name>
</author>
<author><name sortKey="Heise, M" uniqKey="Heise M">M Heise</name>
</author>
<author><name sortKey="Baric, R" uniqKey="Baric R">R Baric</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Van Hemert, Mj" uniqKey="Van Hemert M">MJ van Hemert</name>
</author>
<author><name sortKey="Worm, Shvan Den" uniqKey="Worm S">SHvan den Worm</name>
</author>
<author><name sortKey="Knoops, K" uniqKey="Knoops K">K Knoops</name>
</author>
<author><name sortKey="Mommaas, Am" uniqKey="Mommaas A">AM Mommaas</name>
</author>
<author><name sortKey="Gorbalenya, Ae" uniqKey="Gorbalenya A">AE Gorbalenya</name>
</author>
<author><name sortKey="Snijder, Ej" uniqKey="Snijder E">EJ Snijder</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Frieman, M" uniqKey="Frieman M">M Frieman</name>
</author>
<author><name sortKey="Yount, B" uniqKey="Yount B">B Yount</name>
</author>
<author><name sortKey="Heise, M" uniqKey="Heise M">M Heise</name>
</author>
<author><name sortKey="Kopecky Bromberg, Sa" uniqKey="Kopecky Bromberg S">SA Kopecky-Bromberg</name>
</author>
<author><name sortKey="Palese, P" uniqKey="Palese P">P Palese</name>
</author>
<author><name sortKey="Baric, Rs" uniqKey="Baric R">RS Baric</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ye, Y" uniqKey="Ye Y">Y Ye</name>
</author>
<author><name sortKey="Hauns, K" uniqKey="Hauns K">K Hauns</name>
</author>
<author><name sortKey="Langland, Jo" uniqKey="Langland J">JO Langland</name>
</author>
<author><name sortKey="Jacobs, Bl" uniqKey="Jacobs B">BL Jacobs</name>
</author>
<author><name sortKey="Hogue, Bg" uniqKey="Hogue B">BG Hogue</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Spiegel, M" uniqKey="Spiegel M">M Spiegel</name>
</author>
<author><name sortKey="Pichlmair, A" uniqKey="Pichlmair A">A Pichlmair</name>
</author>
<author><name sortKey="Muhlberger, E" uniqKey="Muhlberger E">E Muhlberger</name>
</author>
<author><name sortKey="Haller, O" uniqKey="Haller O">O Haller</name>
</author>
<author><name sortKey="Weber, F" uniqKey="Weber F">F Weber</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fraser, C" uniqKey="Fraser C">C Fraser</name>
</author>
<author><name sortKey="Riley, S" uniqKey="Riley S">S Riley</name>
</author>
<author><name sortKey="Anderson, Rm" uniqKey="Anderson R">RM Anderson</name>
</author>
<author><name sortKey="Ferguson, Nm" uniqKey="Ferguson N">NM Ferguson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Anderson, Rm" uniqKey="Anderson R">RM Anderson</name>
</author>
<author><name sortKey="Fraser, C" uniqKey="Fraser C">C Fraser</name>
</author>
<author><name sortKey="Ghani, Ac" uniqKey="Ghani A">AC Ghani</name>
</author>
<author><name sortKey="Donnelly, Ca" uniqKey="Donnelly C">CA Donnelly</name>
</author>
<author><name sortKey="Riley, S" uniqKey="Riley S">S Riley</name>
</author>
<author><name sortKey="Ferguson, Nm" uniqKey="Ferguson N">NM Ferguson</name>
</author>
<author><name sortKey="Leung, Gm" uniqKey="Leung G">GM Leung</name>
</author>
<author><name sortKey="Lam, Th" uniqKey="Lam T">TH Lam</name>
</author>
<author><name sortKey="Hedley, Aj" uniqKey="Hedley A">AJ Hedley</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Peiris, Js" uniqKey="Peiris J">JS Peiris</name>
</author>
<author><name sortKey="Chu, Cm" uniqKey="Chu C">CM Chu</name>
</author>
<author><name sortKey="Cheng, Vc" uniqKey="Cheng V">VC Cheng</name>
</author>
<author><name sortKey="Chan, Ks" uniqKey="Chan K">KS Chan</name>
</author>
<author><name sortKey="Hung, If" uniqKey="Hung I">IF Hung</name>
</author>
<author><name sortKey="Poon, Ll" uniqKey="Poon L">LL Poon</name>
</author>
<author><name sortKey="Law, Ki" uniqKey="Law K">KI Law</name>
</author>
<author><name sortKey="Tang, Bs" uniqKey="Tang B">BS Tang</name>
</author>
<author><name sortKey="Hon, Ty" uniqKey="Hon T">TY Hon</name>
</author>
<author><name sortKey="Chan, Cs" uniqKey="Chan C">CS Chan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Thiel, V" uniqKey="Thiel V">V Thiel</name>
</author>
<author><name sortKey="Ivanov, Ka" uniqKey="Ivanov K">KA Ivanov</name>
</author>
<author><name sortKey="Putics, A" uniqKey="Putics A">A Putics</name>
</author>
<author><name sortKey="Hertzig, T" uniqKey="Hertzig T">T Hertzig</name>
</author>
<author><name sortKey="Schelle, B" uniqKey="Schelle B">B Schelle</name>
</author>
<author><name sortKey="Bayer, S" uniqKey="Bayer S">S Bayer</name>
</author>
<author><name sortKey="Weissbrich, B" uniqKey="Weissbrich B">B Weissbrich</name>
</author>
<author><name sortKey="Snijder, Ej" uniqKey="Snijder E">EJ Snijder</name>
</author>
<author><name sortKey="Rabenau, H" uniqKey="Rabenau H">H Rabenau</name>
</author>
<author><name sortKey="Doerr, Hw" uniqKey="Doerr H">HW Doerr</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Matrosovich, M" uniqKey="Matrosovich M">M Matrosovich</name>
</author>
<author><name sortKey="Matrosovich, T" uniqKey="Matrosovich T">T Matrosovich</name>
</author>
<author><name sortKey="Garten, W" uniqKey="Garten W">W Garten</name>
</author>
<author><name sortKey="Klenk, Hd" uniqKey="Klenk H">HD Klenk</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chen, L" uniqKey="Chen L">L Chen</name>
</author>
<author><name sortKey="Gui, C" uniqKey="Gui C">C Gui</name>
</author>
<author><name sortKey="Luo, X" uniqKey="Luo X">X Luo</name>
</author>
<author><name sortKey="Yang, Q" uniqKey="Yang Q">Q Yang</name>
</author>
<author><name sortKey="Gunther, S" uniqKey="Gunther S">S Gunther</name>
</author>
<author><name sortKey="Scandella, E" uniqKey="Scandella E">E Scandella</name>
</author>
<author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name>
</author>
<author><name sortKey="Bai, D" uniqKey="Bai D">D Bai</name>
</author>
<author><name sortKey="He, X" uniqKey="He X">X He</name>
</author>
<author><name sortKey="Ludewig, B" uniqKey="Ludewig B">B Ludewig</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Niwa, H" uniqKey="Niwa H">H Niwa</name>
</author>
<author><name sortKey="Yamamura, K" uniqKey="Yamamura K">K Yamamura</name>
</author>
<author><name sortKey="Miyazaki, J" uniqKey="Miyazaki J">J Miyazaki</name>
</author>
</analytic>
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